狂犬病病毒CVS-11株的培养及其效价测定

本研究通过神经瘤细胞（NA）培养CVS-11株狂犬病病毒,并测定不同批次的病毒毒价,为优化狂犬病疫苗的生产工艺提供数据支持,具有实际意义。病毒培养和检测结果表明,第1次和第2次收获的病毒液病毒含量较高,在107.0FFU/0.1 mL以上,毒力为106.4～107.0LD50/0.03 mL;而第3次收获的病毒含量则较低,只有106.0FFU/0.1 mL左右,毒力为105.0～105.3LD50/0.03 mL。第1次和第2次收获的病毒液可用于制备狂犬疫苗,第3次收获的病毒液因毒价较低不适于制备狂犬疫苗。     

Effect of dietary defatted diatom biomass on egg production and quality of laying hens

Background： This study was to determine if feeding laying hens with defatted diatom microalgal biomass （DFA） from biofuel production affected their egg production and health status. Methods： Five replicates of 5 individually caged ISA Babcock White leghorn hens were fed 4 diets, including a corn-soybean meal control diet, a diet containing 7.5% DFA substituting for soybean meal, and diets containing 7.5% or 15% DFA substituting for corn and soybean meal. Body weights, feed intake, feed conversion ratio （FCR）, rate of egg production, egg size, egg mass, and several characteristics of eggs were determined at 4 and 8 wk. Venous blood was sampled at 4 and 8 wk for measurement of 5 biomarkers of health. Results： The15% DFA diet decreased （P 〈 0.05） feed intake, egg production, and plasma uric acid concentrations as compared with the control diet, but increased （P 〈 0.05） egg albumen weight and height compared with the 7.5% DFA diets. The two levels of DFA produced dose-dependent （P 〈 0.05） changes in three color measures of egg yolk, without affecting four hen plasma biochemical indicators of health. Conclusions： Feeding laying hens with 7.5% DFA in the corn-soybean meal diet for 8 wk had no adverse effect on their health, egg production, or egg quality, but 15% inclusion reduced feed intake, egg production, and efficiency of feed utilization.     

基于双次Otsu算法的野外荔枝多类色彩目标快速识别

针对目前野外环境下多类目标识别速度偏慢,导致机器人视觉定位精度低和工作效率不高的难题,以野外环境下成熟荔枝的多类色彩目标识别为例,提出了一种双次Otsu分割算法对多类色彩目标进行识别。首先为了提高算法的效率,改进了传统的Otsu算式;然后对目标色彩图像的背景、果梗、果实分别用改进的Otsu算法进行粗分割和细分割。最后通过与K-均值聚类(K-means)算法、模糊C均值聚类(FCM)算法、Otsu和K-means结合算法、Otsu和FCM结合算法这4种算法进行对比,双次Otsu算法从分割质量及其正确分割率、运行时间、稳定性3方面都优于其他4种算法。实验结果表明,双次Otsu算法对色彩目标的成熟荔枝识别的时间少于0.2 s。     

干燥机粮层通风阻力特性数值模拟与试验

粮层内部通风阻力特性是影响干燥均匀性和能量损耗的关键因素之一。针对传统的经验公式存在精度偏低、未考虑粮层多孔介质的复杂变化因素和非线性的不确定性问题,基于传统的Ergun模型进行了压力损失因素分析;在通风阻力试验平台测定试验数据,通过探索性分析方法对风速进行分段,引入误差影响因子λ,导出了一种新的基于多孔介质Ergun的稻谷变层压力场模型;给出了优化阻力特性曲线,构建了变层阻力与层厚的数学关系。试验结果证实,风速小于0.2m/s时,λ取值为1;风速在0.2~0.4m/s范围内时,λ取值为0.89;风速在0.4~0.6m/s范围内时,λ取值为0.79。     

大棚苦瓜白粉病田间药效试验

开展了四氟醚唑、醚菌酯、丙环·醚菌酯、甲基硫菌灵4种药剂防治大棚苦瓜白粉病的田间药效试验。试验结果表明,4种药剂均可有效控制大棚苦瓜白粉病发展,且对苦瓜安全无药害,以32%丙环·嘧菌酯悬浮剂1 500倍液防效最好,其次是4%四氟醚唑水乳剂1 000倍液、50%醚菌酯水分散粒剂2 000倍液、70%甲基硫菌灵可湿性粉剂600倍液,在生产上防治时可交替使用。     

一个农牧结合生态系统物流模型的建立及持续性分析

建立了人口、玉米连作与奶牛养殖相结合的农牧生态系统物流模型。并运用Ｃ语文编制程序进行预测，经多年实验数据检验效果较好，说明模型有效、可行并具有一定通用性。最后将模型用于哈尔滨玉米带，探索几种不同的农业类型的结构与功能，并进行持续性分析。     

海南强化品种与品牌意识

5月20日，海南省西瓜专业技术协会成立。“琼岛西瓜王”的文昌人梁亚宽出任会长。     

包装玉米长期安全储藏试验

在华南地区高温高湿环境下，在尚不具备机械通风设备的楼房仓内进行玉米包装大堆保管。采用合理的剂量和熏蒸方法，保持粮堆内较长时间的PH3有效浓度，抑制了玉米的呼吸强度和微生物、害虫的生长繁殖，确保了玉米长期安全储藏，保持了较好的储粮品质。     

农业标准化监测管理体系设计

农业标准化是我国农业发展的一项重要内容。为确保农业标准化得以顺利实施，结合上海市农业标准化的实际情况，初步设计了农业标准化监测管理体系，并进一步探讨了其基本运作规律。     

Effects of level of dietary protein and exercise on growth rates of horses

Rates of growth were measured in two-year-old (Experiment 1) and nine-month-old (Experiment 2) horses fed high (12 or 14 per cent) or low (6 or 8 per cent) crude protein diets with one of two levels of exercise (0 or 12 km trotting per day at 12 km/h). In the non-exercised horses feed intakes and growth rates were greater on the high than on the low protein diets. Exercise increased feed intakes and growth rates of horses on the low but not the high protein diets, so that in the exercised groups there were no significant differences in feed intakes or growth rates between the horses on the two dietary protein levels. Exercise increased the apparent digestibility of dry matter, energy and crude protein. There were no differences in skeletal characteristics caused by either dietary protein level or exercise. These results suggest that the growing horse, if exercised regularly, may be given a diet of lower protein concentration than currently recommended without significant reduction in growth rate.